Description

Animal-free, defined 3D cell culture environment
100% plant-derived Re:ancel Bio Matrix

The New Standard for Animal-Free Scaffolds

• Re:ancel Bio Matrix provides cell-friendly properties as a non-animal-derived material.

• Achieve more precise and reproducible 3D cell culture without the compositional complexity and batch-to-batch variability of animal-derived matrices.

Specifications

How to Use

How to Use - Video

Product Information

Versatile Applications of Re:ancel Bio-CNF to Enhance Flexibility in Experimental Design

Re:ancel Bio is a 100% plant-derived, animal-free material, and it has been demonstrated to support a variety of culture methods that can be flexibly adapted to the specific objectives of researchers.

• Embedded Method: A standard protocol in which cells are mixed with Bio-CNF to promote three-dimensional growth within the matrix.

• On-top Method: Cells are seeded on top of a pre-formed Bio-CNF hydrogel layer, enabling the observation of interfacial behavior and structural formation.

Similar to conventional systems such as Matrigel, optimal culture models can be designed according to experimental conditions and cell line characteristics.

Precise 3D Network Design Through Nanostructure Control

Re:ancel Bio enables the design of distinct cellular growth environments by finely tuning the length of nanofibers, even within the same chemical composition.

Superior Cell Viability of Re:ancel Bio Compared to 2D Culture

It has been confirmed that 3D cultures using Re:ancel show significantly higher cell viability compared to conventional 2D culture methods across various cell lines, including HepG2, Huh-7, and PANC-1.

Comparison of 3D Cell Culture Morphology by Re:ancel Bio Type (Day 7 ~ 9)

Depending on the type of Re:ancel Bio, spheroids with diverse morphologies are formed, ranging from grape-like clusters (Type I) to smooth spherical structures (Type III).

Storage condition

Please store at 2 ~ 8 ℃.

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